This is the first study done in Vietnam on anti-inflammatory, analgesic effect of bee venom on experimental animals.
In 2010, in one time participating in research on propolis at beekeeping farms in Japan, Assoc.Prof.Dr. Nguyen Thi Thanh Mai , Head of Department of Chemistry - Head of Department of Chemistry, University of Natural Sciences - University National Ho Chi Minh City, accidentally known bee venom. From there, she and her colleagues began to research deeply about the process of extracting bee venom and conduct research project ' Establishment of process of extracting venom and assessing pharmacological effects in the direction of using support. arthritis treatment aid. ' This topic recently won the second prize of 44 HCMC Innovation Awards 2019.
According to the research team, since ancient times, bee venom (apis mellifera) has been used in folk remedies to treat a number of diseases, most of which are rheumatic pain. Modern studies have shown that bee venom is anti-inflammatory and analgesic because its components contain enzymes, peptides and amines.
In the world, there are also many studies showing that in addition to effective arthritis treatment, bee venom also works to treat a number of other diseases such as pain relief, cancer, high blood pressure, asthma, detoxification. tobacco addiction, drugs, skin beauty, anti-aging .
Currently, beekeeping farms in the world often combine beekeeping for honey and extract of bee venom to make medicine or cosmetics, while in our country, beekeepers only take honey, not recover the source of bee venom. this value. Therefore, the research team has set up the process of extracting, evaluating and analyzing the quality of bee venom from the common honey bee cultured in Vietnam, Apis mellifera .
At first success, the research team boldly presented the topic to Department of Science and Technology of Ho Chi Minh City and it was approved. After that, the group cooperated with Ho Chi Minh City Institute of Ethnic Medicine and Pharmacy to develop the project with the goal of producing injections.
Assoc.Prof.Dr Nguyen Thi Thanh Mai.(Photo: Ho Chi Minh City National University).
The bee venom is extracted by the BVC 0412 bee venom collecting device based on the principle of electric shock by direct current in the form of a capacitor on a bare wire, the venom receiver is a smooth glass. The bare wires are both responsible for electric shocks, and they are a place for bees to cling and crouch to make venom. The glass is responsible for both collecting the venom and ensuring the bee does not lose the needle after the secretion. Then remove the glass, you will see the white crystals on the glass. Use a sharp, thin knife to scrape away this portion of the crystal to get raw bee venom. The raw bee venom after collection from the farm was dissolved in distilled water, shaking vigorously for 30 minutes (300 rpm). Filter the solution through a cellulose filter funnel with a pore size of 20 .m. Solution after filtration is made to coagulate at temperature o C.
Bee venom after freezing has a fine, very light powder, so when conducting freezing, you need to use a covering film with holes in the mouth of the container. After being cleaned, the venom samples were solid, white, preserved at a temperature of -4 o C. The refining efficiency was 93.59%. After the purification of the bee venom, thin chromatography was compared to the quality of the standard venom, the results showed the similarity of the main components between the refined bee venom and the standard venom.
The results also show that the content of the main components in the extracted bee venom is similar to the standard bee venom of Sigma-Aldrich and other countries in the world. Studies evaluating acute and sub-chronic toxicity of bee venom on experimental animals along with studies evaluating anti-arthritis and analgesic effects of bee venom on experimental animals were also conducted. Accordingly, the venom with the dose of 1.0 and 1.5 mg kg-1 has anti-inflammatory, analgesic, reducing the temperature in the foot of the mouse, slightly reducing the amount of white blood cells and red blood cells in the peripheral blood during 21 days after causing inflammation. In addition, the effect of bee venom dose of 1.5 mg kg-1 is similar to mobic dose of 1.0 mg kg-1 in mice.
The study also adds a database for further in-depth studies to put bee venom into use in pharmaceutical or cosmetics.
According to Assoc.Prof.Dr. Nguyen Thi Thanh Mai, depending on the type of disease and patients (through close examination and testing), a certain mixture of bee doses (Apitoxin: Unité d 'apitoxine according to Ioirich, 1ml = 5 applications) is used. taste) with novocaine or vitamin C in combination with novocaine to hydrate on the indicated meridians of the meridians (not where the pain is). Research conducted on 156 patients (cured in four years) showed that: patients with rheumatoid arthritis less painful, less swelling; minor asthma attacks, episodes, or mild attacks; Peripheral nerves that have been paralyzed can recover function; Non-fluctuating blood pressure; patients get relief from headaches, sleep well .
Assoc.TSNguyen Thi Thanh Mai et al.
Currently, the group is studying to prepare, evaluate the anti-inflammatory analgesic effect of the magnet injected drug (injected into the acupuncture point) and rubbing in addition to bee venom extract in experiments and in patients with back pain, shoulder arthritis and regressions chemistry of knee joints to develop anti-arthritis medication from bee venom, which has been used in folklore but has no scientific basis. This is the first study done in Vietnam on the process of extracting, refining, analyzing ingredients, developing a base quality standard of bee venom as well as assessing acute and semi-toxic toxicity. demonstration and anti-inflammatory, analgesic effect of bee venom on experimental animals.
"In the world, the process to create a new drug takes 10-20 years. Therefore, we are still on the way of researching, developing the process of drug preparation, dosage, storage and evaluation. With support from VNU-HCM, Ho Chi Minh City Department of Science and Technology, the group is looking for investment from businesses to commercialize products.We divide the process into several small stages, completing each stage into stages. At the end, I would like you to join the business " , Associate Professor Mai shared.