Application of tissue culture to preserve orchids of Kim Diep

Researchers at Hue University have applied tissue culture technology (in vitro) to increase the multiplication coefficient from Golden Orchid orchids, which can create from a few thousand to 1 million seedlings.

Succeeding in increasing the number of endangered species of Kim Diep orchids is an important contribution to the conservation of this rare genetic species; At the same time, creating an important source of raw materials for the production of Kim Diep orchids to contribute to economic development.

The process is started when the three-month-old Kim Diep orchid is harvested from natural plants, then soaked in diluted soapy water and thoroughly washed under running water. Sterilize orchids with 70% alcohol for two minutes and rinse them five times with sterile distilled water.

Orchid

Within the first two weeks, the seed is grown in tissue culture that turns yellowish and begins to grow. From the next three to four weeks, the seeds continue to grow and sprout, after being cultured in medium with moderate growth stimulants.

Then the shoots are separated and transplanted in the medium to create roots. By the time the seedlings are fully regenerated, 3cm to 4.5cm tall and two to five roots are transferred from the culture room to the natural environment that qualifies for the plant to adapt gradually. And when transferring seedlings out of culture tanks, clean the environment, cut roots and dip them into antiseptic solution.

Seedlings should be planted on the moss and ferns, cover the light about 50% and sprinkle water. Two weeks later, the seedlings will begin to form new roots and form leaves after three weeks of age. In the natural nursery, the rate of live Kim Diep orchids reaches nearly 91%.

According to researchers, Kim Diep is a wild orchid species mainly distributed in the Central Highlands of Vietnam. Lan Kim Diep has large flowers, bright yellow.

However, Kim Diep orchids are in the danger group and are at risk of extinction. The reason is that Kim Diep orchids grow very poor even when ripe. At the same time, seeds depend on fungal infections and depend entirely on natural conditions for germination.

On the other hand, Kim Diep orchids are very rare, so people have over-exploited, along with the increasingly narrow forest area . which has made the source of this species of orchids increasingly exhausted.